Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Potassium channel subfamily K member 2 (also known as KCNK2) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse KCNK2 antibody. KCNK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse KCNK2 Antibody is added and binds to KCNK2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KCNK2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse KCNK2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm